AgraQuant® Ochratoxin ELISA
AgraQuant® OTA test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. Together with a StatFax® or BioTek® ELISA reader, results can be directly interpreted and continuously documented. These ELISA test kits are the ideal solution for a parallel measurement of multiple samples with incubation times of as low as 15 minutes for up to 42 samples. With the AgraQuant® ELISA, up to 6 mycotoxins can be analyzed from 1 extraction, substantially shortening overall time-to-result.
| Quantitation range | 2-40 ppb |
|---|---|
| Limit of detection | 1.9 ppb |
| Limit of quantitation | 2 ppb |
| Technology/Application | ELISA |
| Storage Temperature | 2°C – 8°C |
| Result type | Quantitative |
| Product/Environment | Product Testing |
Materials supplied in the standard product
- antibody coated microwells in a microwell holder
- non-coated dilution microwells
- 5 vials of each ochratoxin A standard
- 1 bottle of ochratoxin A conjugate
- 1 bottle of substrate solution
- 1 bottle of stop solution
Materials required but not supplied
- Romer Series II® Mill or equivalent
- balance – 400 g
- blender or a tightly sealing jar with lid
- 250mL conic flask with plug
- graduated cylinder: 100mL
- 100% methanol: ACS grade methanol
- 70% methanol
- distilled or de-ionized water
- container for filtrate collection (e.g. flask or falcon tubes)
- Whatman#1 filter paper or equivalent
- filter funnel
- 8-channel and single channel pipettes capable of pipetting 100µL and 200µL with tips
- timer
- wash bottle
- absorbent paper towels
- 3 reagent boats for 8-channel pipettes
- microwell reader with a 450nm filter and an optional differential filter of 630nm or equivalent
