AgraQuant® T-2/HT-2 ELISA
The AgraQuant® T-2/HT-2 test kit is an accurate and reliable enzyme-linked immunosorbent assay (ELISA) in a quantitative format. Used in conjunction with an ELISA reader, such as the Byonoy Absorbance 96 or the BioTek model, it enables direct interpretation and continuous documentation of results. AgraQuant® T-2/HT-2 ELISA test kits are ideal for parallel measurement of multiple samples, offering incubation times as low as 20 minutes for up to 42 samples.
| Quantitation range | 24-500 ppb |
|---|---|
| Limit of detection | 24 ppb |
| Limit of quantitation | 24 ppb |
| Mycotoxins | T-2/HT-2 |
| Technology/Application | ELISA |
| Storage Temperature | 2°C – 8°C |
| Result type | Quantitative |
| Product/Environment | Product Testing |
Materials supplied in the standard product
- antibody coated microwells in a microwell holder
- non-coated dilution microwells
- 5 vials of T-2 /HT-2 standard (0; 24; 120; 240 and 500 ppb)
- 1 green-capped bottle of T-2/HT-2 toxin-conjugate solution
- 1 blue-capped bottle of substrate solution
- 1 red-capped bottle of stop solution
Materials required but not supplied
- grinding mill
- balance – 400 g
- blender or a tightly sealing jar with lid
- graduated cylinder: 100mL
- 50% Methanol (50:50 methanol:water) or
- Methanol (ACS grade) and distilled or deionized water
- container for filtrate collection with minimum 125mL capacity
- Whatman#1 filter paper or equivalent
- filter funnel
- 8-channel and single channel pipettes capable of pipetting 100µL and 200µL with tips
- timer
- wash bottle
- absorbent paper towels
- 3 reagent boats for 8-channel pipettes
- microwell reader with 450 nm and 630 nm filters
